Fractionation and characterization of urinary filarial antigen in bancroftian filariasis.
Identifieur interne : 00E638 ( Main/Exploration ); précédent : 00E637; suivant : 00E639Fractionation and characterization of urinary filarial antigen in bancroftian filariasis.
Auteurs : P. Rama Prasad [Inde] ; B C HarinathSource :
- Asian Pacific journal of allergy and immunology [ 0125-877X ] ; 1987.
Descripteurs français
- KwdFr :
- Albuminurie (immunologie), Animaux, Anticorps antihelminthe (analyse), Antigènes d'helminthe (immunologie), Antigènes d'helminthe (urine), Filariose lymphatique (immunologie), Filariose lymphatique (urine), Filarioses (urine), Glycoprotéines (immunologie), Glycoprotéines (urine), Humains, Microfilaria (immunologie), Test ELISA, Wuchereria (immunologie), Wuchereria bancrofti (immunologie).
- MESH :
- analyse : Anticorps antihelminthe.
- immunologie : Albuminurie, Antigènes d'helminthe, Filariose lymphatique, Glycoprotéines, Microfilaria, Wuchereria, Wuchereria bancrofti.
- urine : Animaux, Antigènes d'helminthe, Filariose lymphatique, Filarioses, Glycoprotéines, Humains, Test ELISA.
English descriptors
- KwdEn :
- Albuminuria (immunology), Animals, Antibodies, Helminth (analysis), Antigens, Helminth (immunology), Antigens, Helminth (urine), Elephantiasis, Filarial (immunology), Elephantiasis, Filarial (urine), Enzyme-Linked Immunosorbent Assay, Filariasis (urine), Glycoproteins (immunology), Glycoproteins (urine), Humans, Microfilariae (immunology), Wuchereria (immunology), Wuchereria bancrofti (immunology).
- MESH :
- chemical , analysis : Antibodies, Helminth.
- immunology : Albuminuria, Antigens, Helminth, Elephantiasis, Filarial, Glycoproteins, Microfilariae, Wuchereria, Wuchereria bancrofti.
- chemical , urine : Antigens, Helminth, Elephantiasis, Filarial, Filariasis, Glycoproteins.
- Animals, Enzyme-Linked Immunosorbent Assay, Humans.
Abstract
Urine samples from microfilaraemic patients were concentrated and fractionated by gel chromatography on Ultrogel AcA 44. Four protein fractions labelled as UFA C1, UFA C2, UFA C3 and UFA C4 were tested for filarial antigenicity by sandwich ELISA. UFA C1 and UFA C2 showed antigenic activity. On further analysis by SDS-PAGE, UFA C1 and UFA C2 showed antigenic components with MW ranging from 10.4 K to 123 K. UFA C1-1 and UFA C2-2 showed high antigen titre in ELISA. Urinary albumin was observed as a major component in UFA C2. Absorption of albumin from UFA C2 enhanced its antigenic activity considerably. As little as 0.01 pg antigenic protein per test was found to be sufficient for the detection of filarial antibody in ELISA. Biochemical characterization indicated a glycoprotein nature of UFA C2.
PubMed: 3329520
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 006316
- to stream PubMed, to step Curation: 006316
- to stream PubMed, to step Checkpoint: 006316
- to stream Ncbi, to step Merge: 009740
- to stream Ncbi, to step Curation: 009740
- to stream Ncbi, to step Checkpoint: 009740
- to stream Main, to step Merge: 00F351
- to stream Main, to step Curation: 00E638
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Fractionation and characterization of urinary filarial antigen in bancroftian filariasis.</title><author><name sortKey="Rama Prasad, P" sort="Rama Prasad, P" uniqKey="Rama Prasad P" first="P" last="Rama Prasad">P. Rama Prasad</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry Mahatma Gandhi Institute of Medical Sciences, Sevagram, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biochemistry Mahatma Gandhi Institute of Medical Sciences, Sevagram</wicri:regionArea><wicri:noRegion>Sevagram</wicri:noRegion></affiliation></author><author><name sortKey="Harinath, B C" sort="Harinath, B C" uniqKey="Harinath B" first="B C" last="Harinath">B C Harinath</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1987">1987</date><idno type="RBID">pubmed:3329520</idno><idno type="pmid">3329520</idno><idno type="wicri:Area/PubMed/Corpus">006316</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">006316</idno><idno type="wicri:Area/PubMed/Curation">006316</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">006316</idno><idno type="wicri:Area/PubMed/Checkpoint">006316</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">006316</idno><idno type="wicri:Area/Ncbi/Merge">009740</idno><idno type="wicri:Area/Ncbi/Curation">009740</idno><idno type="wicri:Area/Ncbi/Checkpoint">009740</idno><idno type="wicri:doubleKey">0125-877X:1987:Rama Prasad P:fractionation:and:characterization</idno><idno type="wicri:Area/Main/Merge">00F351</idno><idno type="wicri:Area/Main/Curation">00E638</idno><idno type="wicri:Area/Main/Exploration">00E638</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Fractionation and characterization of urinary filarial antigen in bancroftian filariasis.</title><author><name sortKey="Rama Prasad, P" sort="Rama Prasad, P" uniqKey="Rama Prasad P" first="P" last="Rama Prasad">P. Rama Prasad</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry Mahatma Gandhi Institute of Medical Sciences, Sevagram, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biochemistry Mahatma Gandhi Institute of Medical Sciences, Sevagram</wicri:regionArea><wicri:noRegion>Sevagram</wicri:noRegion></affiliation></author><author><name sortKey="Harinath, B C" sort="Harinath, B C" uniqKey="Harinath B" first="B C" last="Harinath">B C Harinath</name></author></analytic><series><title level="j">Asian Pacific journal of allergy and immunology</title><idno type="ISSN">0125-877X</idno><imprint><date when="1987" type="published">1987</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Albuminuria (immunology)</term><term>Animals</term><term>Antibodies, Helminth (analysis)</term><term>Antigens, Helminth (immunology)</term><term>Antigens, Helminth (urine)</term><term>Elephantiasis, Filarial (immunology)</term><term>Elephantiasis, Filarial (urine)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Filariasis (urine)</term><term>Glycoproteins (immunology)</term><term>Glycoproteins (urine)</term><term>Humans</term><term>Microfilariae (immunology)</term><term>Wuchereria (immunology)</term><term>Wuchereria bancrofti (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Albuminurie (immunologie)</term><term>Animaux</term><term>Anticorps antihelminthe (analyse)</term><term>Antigènes d'helminthe (immunologie)</term><term>Antigènes d'helminthe (urine)</term><term>Filariose lymphatique (immunologie)</term><term>Filariose lymphatique (urine)</term><term>Filarioses (urine)</term><term>Glycoprotéines (immunologie)</term><term>Glycoprotéines (urine)</term><term>Humains</term><term>Microfilaria (immunologie)</term><term>Test ELISA</term><term>Wuchereria (immunologie)</term><term>Wuchereria bancrofti (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antibodies, Helminth</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Anticorps antihelminthe</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Albuminurie</term><term>Antigènes d'helminthe</term><term>Filariose lymphatique</term><term>Glycoprotéines</term><term>Microfilaria</term><term>Wuchereria</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Albuminuria</term><term>Antigens, Helminth</term><term>Elephantiasis, Filarial</term><term>Glycoproteins</term><term>Microfilariae</term><term>Wuchereria</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" type="chemical" qualifier="urine" xml:lang="en"><term>Antigens, Helminth</term><term>Elephantiasis, Filarial</term><term>Filariasis</term><term>Glycoproteins</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Humans</term></keywords><keywords scheme="MESH" qualifier="urine" xml:lang="fr"><term>Animaux</term><term>Antigènes d'helminthe</term><term>Filariose lymphatique</term><term>Filarioses</term><term>Glycoprotéines</term><term>Humains</term><term>Test ELISA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Urine samples from microfilaraemic patients were concentrated and fractionated by gel chromatography on Ultrogel AcA 44. Four protein fractions labelled as UFA C1, UFA C2, UFA C3 and UFA C4 were tested for filarial antigenicity by sandwich ELISA. UFA C1 and UFA C2 showed antigenic activity. On further analysis by SDS-PAGE, UFA C1 and UFA C2 showed antigenic components with MW ranging from 10.4 K to 123 K. UFA C1-1 and UFA C2-2 showed high antigen titre in ELISA. Urinary albumin was observed as a major component in UFA C2. Absorption of albumin from UFA C2 enhanced its antigenic activity considerably. As little as 0.01 pg antigenic protein per test was found to be sufficient for the detection of filarial antibody in ELISA. Biochemical characterization indicated a glycoprotein nature of UFA C2.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Harinath, B C" sort="Harinath, B C" uniqKey="Harinath B" first="B C" last="Harinath">B C Harinath</name></noCountry><country name="Inde"><noRegion><name sortKey="Rama Prasad, P" sort="Rama Prasad, P" uniqKey="Rama Prasad P" first="P" last="Rama Prasad">P. Rama Prasad</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 00E638 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 00E638 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:3329520
|texte= Fractionation and characterization of urinary filarial antigen in bancroftian filariasis.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:3329520" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |